HBEGF Antibody / DT-R

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NSJ Bioreagents
Product Code: NSJ-RQ7309
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7309-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HBEGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with HBEGF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 7
IHC staining of FFPE human appendiceal adenocarcinoma tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human lung adenocarcinoma tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE mouse lung tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE rat respiratory smooth muscle tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
Western blot testing of 1) human HeLa, 2) human K562, 3) human Jurkat, 4) human U-251, 5) rat C6 and 6) mouse lung tissue lysate with HBEGF antibody. Predicted molecular weight ~23 kDa.
7 / 7
Flow cytometry testing of human Jurkat cells with HBEGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HBEGF antibody.
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Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with HBEGF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lung tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat respiratory smooth muscle tissue with HBEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human K562, 3) human Jurkat, 4) human U-251, 5) rat C6 and 6) mouse lung tissue lysate with HBEGF antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human Jurkat cells with HBEGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HBEGF antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the HBEGF antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Heparin-binding EGF-like growth factor (HB-EGF), also called Diphtheria toxin receptor (DT-R), is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. Enables growth factor activity and heparin binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; and positive regulation of wound healing. Located in cell surface and extracellular space. Implicated in glomerulosclerosis and perinatal necrotizing enterocolitis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KKRDPCLRKYKDFCIHGECKYVKE were used as the immunogen for the HBEGF antibody.
Limitation:
This HBEGF antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q99075