MSX2 Antibody / Hox-8

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NSJ Bioreagents
Product Code: NSJ-RQ7311
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7311-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the MSX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human RT4 cells with MSX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSX2 antibody.
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Flow cytometry testing of human PC-3 cells with MSX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSX2 antibody.
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Western blot testing of human 1) RT4, 2) MCF7 and 3) T-47D cell lysate with MSX2 antibody. Expected molecular weight 29-37 kDa.
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Flow cytometry testing of human RT4 cells with MSX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSX2 antibody.
Flow cytometry testing of human PC-3 cells with MSX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSX2 antibody.
Western blot testing of human 1) RT4, 2) MCF7 and 3) T-47D cell lysate with MSX2 antibody. Expected molecular weight 29-37 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MSX2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Homeobox protein MSX-2, also called Homeobox protein Hox-8 (Hox8) is a protein that in humans is encoded by the MSX2 gene. This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-M129) was used as the immunogen for the MSX2 antibody.
Limitation:
This MSX2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P35548