ADAR2 Antibody / ADARB1 / RED1

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NSJ Bioreagents
Product Code: NSJ-RQ7318
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7318-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ADAR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
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Flow cytometry testing of human HEL cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
3 / 3
Western blot testing of 1) human SiHa, 2) human HeLa, 3) human HEL, 4) rat brain and 5) mouse brain tissue lysate with ADAR2 antibody. Expected molecular weight: 80-90 kDa.
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Flow cytometry testing of human 293T cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
Flow cytometry testing of human HEL cells with ADAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAR2 antibody.
Western blot testing of 1) human SiHa, 2) human HeLa, 3) human HEL, 4) rat brain and 5) mouse brain tissue lysate with ADAR2 antibody. Expected molecular weight: 80-90 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ADAR2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Double-stranded RNA-specific editase 1 is an enzyme that in humans is encoded by the ADARB1 gene. This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K234-R714) was used as the immunogen for the ADAR2 antibody.
Limitation:
This ADAR2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P78563