C-1-tetrahydrofolate synthase Antibody / MTHFD1

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NSJ Bioreagents
Product Code: NSJ-RQ7319
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7319-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the C-1-tetrahydrofolate synthase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human liver cancer tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human thyroid cancer tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human adenocarcinoma of the lung with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human diffuse large B cell lymphoma tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human chronic tonsillitis tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE rat liver tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
Immunofluorescent staining of FFPE human tonsil tissue with C-1-tetrahydrofolate synthase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
8 / 10
Immunofluorescent staining of FFPE human HeLa cells with C-1-tetrahydrofolate synthase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Western blot testing of 1) human HeLa, 2) human 293T, 3) rat brain, 4) mouse brain and 5) mouse kidney tissue lysate with C-1-tetrahydrofolate synthase antibody. Predicted molecular weight ~102 kDa.
10 / 10
Flow cytometry testing of human JK cells with C-1-tetrahydrofolate synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C-1-tetrahydrofolate synthase antibody.
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IHC staining of FFPE human liver cancer tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adenocarcinoma of the lung with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B cell lymphoma tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human chronic tonsillitis tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with C-1-tetrahydrofolate synthase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human tonsil tissue with C-1-tetrahydrofolate synthase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with C-1-tetrahydrofolate synthase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) rat brain, 4) mouse brain and 5) mouse kidney tissue lysate with C-1-tetrahydrofolate synthase antibody. Predicted molecular weight ~102 kDa.
Flow cytometry testing of human JK cells with C-1-tetrahydrofolate synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C-1-tetrahydrofolate synthase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the C-1-tetrahydrofolate synthase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like). The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K21-Q855) was used as the immunogen for the C-1-tetrahydrofolate synthase antibody.
Limitation:
This C-1-tetrahydrofolate synthase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P11586