Ppp1r15a Antibody / Gadd34

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NSJ Bioreagents
Product Code: NSJ-RQ7322
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7322-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Ppp1r15a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of mouse ANA-1 cells with Ppp1r15a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ppp1r15a antibody.~
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Wester blot testing of mouse stomach tissue lysate with Ppp1r15a anti
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Flow cytometry testing of mouse ANA-1 cells with Ppp1r15a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ppp1r15a antibody.~
Wester blot testing of mouse stomach tissue lysate with Ppp1r15a anti

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ppp1r15a antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. Enables protein phosphatase binding activity and protein phosphatase regulator activity. Involved in several processes, including peptidyl-serine dephosphorylation; regulation of gene expression; and regulation of protein modification process. Acts upstream of or within endoplasmic reticulum unfolded protein response. Predicted to be located in several cellular components, including Golgi apparatus; cytosol; and mitochondrion. Predicted to be part of protein phosphatase type 1 complex. Predicted to be active in endoplasmic reticulum. Is expressed in several structures, including central nervous system; genitourinary system; gut; hemolymphoid system gland; and liver. Used to study thalassemia. Orthologous to human PPP1R15A (protein phosphatase 1 regulatory subunit 15A).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids R14-R572) was used as the immunogen for the Ppp1r15a antibody.
Limitation:
This Ppp1r15a antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P17564