BAG2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7328
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7328-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 8F11G2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the BAG2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human K562 cells with BAG2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BAG2 antibody.~
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Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human HepG2, 4) hum
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Flow cytometry testing of human K562 cells with BAG2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BAG2 antibody.~
Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human HepG2, 4) hum

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the BAG2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-N211) was used as the immunogen for the BAG2 antibody.
Limitation:
This BAG2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O95816