TOMM40 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7332
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7332-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TOMM40 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10
Immunofluorescent staining of FFPE human colon cancer tissue with TOMM40 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 10
Immunofluorescent staining of FFPE human A549 cells with TOMM40 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 10
IHC staining of FFPE human ovarian cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human liver cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human rectal cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human lung adenocarcinoma tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human diffuse large B cell lymphoma tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE human tonsil tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7 and 4) HepG2 cell lysate with TOMM40 antibody. Predicted molecular weight ~37 kDa.
10 / 10
Flow cytometry testing of human MCF7 cells with TOMM40 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOMM40 antibody.
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Immunofluorescent staining of FFPE human colon cancer tissue with TOMM40 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with TOMM40 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human ovarian cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B cell lymphoma tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with TOMM40 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7 and 4) HepG2 cell lysate with TOMM40 antibody. Predicted molecular weight ~37 kDa.
Flow cytometry testing of human MCF7 cells with TOMM40 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOMM40 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TOMM40 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Translocase of outer mitochondrial membrane 40 homolog (yeast), also known as TOMM40, is a protein which in humans is encoded by the TOMM40 gene. The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N111-K315) was used as the immunogen for the TOMM40 antibody.
Limitation:
This TOMM40 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
O96008