STK24 Antibody / Serine/threonine-protein kinase 24 / MST-3

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NSJ Bioreagents
Product Code: NSJ-RQ7343
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7343-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the STK24 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat stomach, 2) rat testis, 3) rat brain, 4) mouse stomach, 5) mouse small intestine, 6) mouse testis and 7) mouse brain tissue lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
2 / 5
Western blot testing of human 1) A431, 2) HeLa, 3) Raji, 4) U-87 MG, 5) 293T, 6) placenta, 7) Caco-2 and 8) RT4 cell lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
3 / 5
IHC staining of FFPE mouse brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE rat brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
Flow cytometry testing of human U-251 cells with STK24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STK24 antibody.
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Western blot testing of 1) rat stomach, 2) rat testis, 3) rat brain, 4) mouse stomach, 5) mouse small intestine, 6) mouse testis and 7) mouse brain tissue lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human 1) A431, 2) HeLa, 3) Raji, 4) U-87 MG, 5) 293T, 6) placenta, 7) Caco-2 and 8) RT4 cell lysate with STK24 antibody. Predicted molecular weight ~49 kDa.
IHC staining of FFPE mouse brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with STK24 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human U-251 cells with STK24 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STK24 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the STK24 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Serine/threonine-protein kinase 24 (STK24), also called Mammalian STE20-like protein kinase 3 (MST-3) is an enzyme that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-Y433) was used as the immunogen for the STK24 antibody.
Limitation:
This STK24 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9Y6E0