17-beta-HSD 3 Antibody / HSD17B3

NSJ Bioreagents
Product Code: NSJ-RQ7344
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7344-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the 17-beta-HSD 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with 17-beta-HSD 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 17-beta-HSD 3 antibody.~
2 / 2
Western blot testing of 1) human U-2 OS, 2) human HeLa, 3)

Flow cytometry testing of human HepG2 cells with 17-beta-HSD 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 17-beta-HSD 3 antibody.~
Western blot testing of 1) human U-2 OS, 2) human HeLa, 3)

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the 17-beta-HSD 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
17beta-Hydroxysteroid dehydrogenase 3 (17b-HSD3) is an enzyme that in humans is encoded by the HSD17B3 gene and is involved in androgen steroidogenesis. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids NLLPSHFLNAPDEIQSLIH were used as the immunogen for the 17-beta-HSD 3 antibody.
Limitation:
This 17-beta-HSD 3 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P37058