CFIm68 Antibody / CPSF6

Product Code: NSJ-RQ7345

Code	Size	Price

NSJ-RQ7345-100UG

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: Mouse IgG1

Antibody Clonality: Monoclonal

Antibody Clone: 3F11E1

Regulatory Status: RUO

Target Species:

Human
Monkey
Mouse
Rat

Applications:

Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the CFIm68 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10

IHC staining of FFPE human colorectal adenocarcinoma tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

2 / 10

IHC staining of FFPE human ovarian cancer tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 10

IHC staining of FFPE human cervical cancer tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 10

IHC staining of FFPE human spleen tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 10

IHC staining of FFPE human squamous cell carcinoma tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 10

IHC staining of FFPE mouse brain tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

7 / 10

IHC staining of FFPE rat brain tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 10

Immunofluorescent staining of FFPE human U-2 OS cells with CFIm68 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

9 / 10

Western blot testing of 1) human HeLa, 2) human 293T, 3) monkey COS-7, 4) rat brain, 5) rat thymus, 6) mouse brain and 7) mouse thymus tissue lysate with CFIm68 antibody. Predicted molecular weight: 52-63 kDa (multiple isoforms).

10 / 10

Flow cytometry testing of human HepG2 cells with CFIm68 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CFIm68 antibody.

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Documents

Datasheet

Further Information

Application Details:

Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells

Application Note:

Optimal dilution of the CFIm68 antibody should be determined by the researcher.

Buffer:

Lyophilized from 1X PBS with 2% Trehalose

Description:

Cleavage and polyadenylation specificity factor subunit 6, also called Cleavage factor Im complex 68 kDa subunit (CFIm68), is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Format:

Antigen affinity purified

Formulation:

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Recombinant human protein (amino acids R50-Q176) was used as the immunogen for the CFIm68 antibody.

Limitation:

This CFIm68 antibody is available for research use only.

Localization:

Cytoplasmic, nuclear

Purity:

Antigen affinity purified

Uniprot #:

Q16630