RGS7 Antibody / Regulator of G-protein signaling 7

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NSJ Bioreagents
Product Code: NSJ-RQ7350
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7350-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the RGS7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human SiHa cells with RGS7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human 293T cells with RGS7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RGS7 antibody.
3 / 3
Western blot testing of 1) human U-87 MG, 2) human SH-SY5Y, 3) human SK-N-SH, 4) human U-251, 5) rat brain and 6) mouse brain tissue lysate with RGS7 antibody. Predicted molecular weight: 49-57 kDa (multiple isoforms).
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Immunofluorescent staining of FFPE human SiHa cells with RGS7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human 293T cells with RGS7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RGS7 antibody.
Western blot testing of 1) human U-87 MG, 2) human SH-SY5Y, 3) human SK-N-SH, 4) human U-251, 5) rat brain and 6) mouse brain tissue lysate with RGS7 antibody. Predicted molecular weight: 49-57 kDa (multiple isoforms).

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RGS7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Regulator of G-protein signaling 7 is a protein that in humans is encoded by the RGS7 gene. Enables G-protein beta-subunit binding activity and GTPase activator activity. Involved in positive regulation of GTPase activity. Located in cytosol.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-N483) was used as the immunogen for the RGS7 antibody.
Limitation:
This RGS7 antibody is available for research use only.
Localization:
Cytoplasm, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
P49802