MAT2A Antibody / Methionine adenosyltransferase 2

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NSJ Bioreagents
Product Code: NSJ-RQ7352
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7352-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MAT2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human lung adenocarcinoma tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human tonsil tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human rectal cancer tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human appendiceal adenocarcinoma tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937, 4) human Daudi, 5) rat kidney and 6) mouse kidney tissue lysate with MAT2A antibody. Predicted molecular weight ~44 kDa.
6 / 6
Flow cytometry testing of human MCF7 cells with MAT2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAT2A antibody.
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IHC staining of FFPE human lung adenocarcinoma tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with MAT2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human U937, 4) human Daudi, 5) rat kidney and 6) mouse kidney tissue lysate with MAT2A antibody. Predicted molecular weight ~44 kDa.
Flow cytometry testing of human MCF7 cells with MAT2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAT2A antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MAT2A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The S-adenosylmethionine synthase isoform type-2 protein catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-A229) was used as the immunogen for the MAT2A antibody.
Limitation:
This MAT2A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P31153