RAB23 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7353
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7353-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB23 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human MCF7 cells with RAB23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB23 antibody.
2 / 3
Flow cytometry testing of human SiHa cells with RAB23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB23 antibody.
3 / 3
Western blot testing of 1) human 293T, 2) human MCF7, 3) human Jurkat, 4) human HepG2, 5) rat brain and 6) mouse brain tissue lysate with RAB23 antibody. Predicted molecular weight: ~27 kDa.
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Flow cytometry testing of human MCF7 cells with RAB23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB23 antibody.
Flow cytometry testing of human SiHa cells with RAB23 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB23 antibody.
Western blot testing of 1) human 293T, 2) human MCF7, 3) human Jurkat, 4) human HepG2, 5) rat brain and 6) mouse brain tissue lysate with RAB23 antibody. Predicted molecular weight: ~27 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RAB23 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ras-related protein Rab-23 is a protein that in humans is encoded by the RAB23 gene. This gene encodes a small GTPase of the Ras superfamily. Rab proteins are involved in the regulation of diverse cellular functions associated with intracellular membrane trafficking, including autophagy and immune response to bacterial infection. The encoded protein may play a role in central nervous system development by antagonizing sonic hedgehog signaling. Disruption of this gene has been implicated in Carpenter syndrome as well as cancer. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K22-K168) was used as the immunogen for the RAB23 antibody.
Limitation:
This RAB23 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9ULC3