HO-2 Antibody / Heme oxygenase 2 / HMOX2

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NSJ Bioreagents
Product Code: NSJ-RQ7367
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7367-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HO-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human appendiceal adenocarcinoma tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human ovary tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human lung squamous cell carcinoma tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human MCF7 cells with HO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of human 1) Jurkat, 2) HeLa, 3) K562 and 4) PANC-1 cell lysate with HO-2 antibody. Predicted molecular weight ~36 kDa.
6 / 6
Flow cytometry testing of human MCF7 cells with HO-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HO-2 antibody.
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IHC staining of FFPE human appendiceal adenocarcinoma tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovary tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung squamous cell carcinoma tissue with HO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with HO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Jurkat, 2) HeLa, 3) K562 and 4) PANC-1 cell lysate with HO-2 antibody. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human MCF7 cells with HO-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HO-2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HO-2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Heme oxygenase 2 (HMOX2), also known as HO-2, is an enzyme that in humans is encoded by the HMOX2 gene. It is mapped to 16p13.3. HMOX2 belongs to the heme oxygenase family. Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H45-A301) was used as the immunogen for the HO-2 antibody.
Limitation:
This HO-2 antibody is available for research use only.
Localization:
Cytoplasmic (ER)
Purity:
Antigen affinity purified
Uniprot #:
P30519