Adgrg1 Antibody / Gpr56 / Adhesion G-protein coupled receptor G1

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NSJ Bioreagents
Product Code: NSJ-RQ7368
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7368-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Adgrg1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE mouse brain tissue with Adgrg1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat brain tissue with Adgrg1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) rat brain, 2) rat heart, 3) rat C6, 4) mouse brain, 5) mouse heart, 6) mouse Neuro-2a and 7) mouse C2C12 cell lysate with Adgrg1 antibody. Predicted molecular weight ~78 kDa.
4 / 4
Flow cytometry testing of mouse RAW264.7 cells with Adgrg1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adgrg1 antibody.
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IHC staining of FFPE mouse brain tissue with Adgrg1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Adgrg1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat heart, 3) rat C6, 4) mouse brain, 5) mouse heart, 6) mouse Neuro-2a and 7) mouse C2C12 cell lysate with Adgrg1 antibody. Predicted molecular weight ~78 kDa.
Flow cytometry testing of mouse RAW264.7 cells with Adgrg1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adgrg1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Adgrg1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables several functions, including collagen binding activity; extracellular matrix binding activity; and heparin binding activity. Involved in several processes, including negative regulation of neuron migration; positive regulation of Rho protein signal transduction; and seminiferous tubule development. Acts upstream of or within cerebral cortex regionalization; hematopoietic stem cell homeostasis; and positive regulation of neural precursor cell proliferation. Located in glial limiting end-foot. Is expressed in several structures, including alimentary system; ganglia; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in bilateral frontoparietal polymicrogyria and bilateral perisylvian polymicrogyria. Orthologous to human ADGRG1 (adhesion G protein-coupled receptor G1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E53-S554) was used as the immunogen for the Adgrg1 antibody.
Limitation:
This Adgrg1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q8K209