17HSD7 Antibody / HSD17B7

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NSJ Bioreagents
Product Code: NSJ-RQ7371
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7371-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 17HSD7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human colorectal adenocarcinoma tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human prostate cancer tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human liver cancer tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human HepG2 cells with 17HSD7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of 1) human HepG2, 2) human HL60, 3) human 293T, 4) human Jurkat, 5) human RT4, 6) rat liver, 7) rat lung and 8) rat RH35 cell lysate with 17HSD7 antibody. Predicted molecular weight ~38 kDa.
6 / 6
Flow cytometry testing of human JK cells with 17HSD7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 17HSD7 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HepG2 cells with 17HSD7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human HL60, 3) human 293T, 4) human Jurkat, 5) human RT4, 6) rat liver, 7) rat lung and 8) rat RH35 cell lysate with 17HSD7 antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human JK cells with 17HSD7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 17HSD7 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the 17HSD7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
3-keto-steroid reductase is an enzyme that in humans is encoded by the HSD17B7 gene. HSD17B7 encodes an enzyme that functions both as a 17-beta-hydroxysteroid dehydrogenase (EC 1.1.1.62) in the biosynthesis of sex steroids and as a 3-ketosteroid reductase (EC 1.1.1.270) in the biosynthesis of cholesterol.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L16-L336) was used as the immunogen for the 17HSD7 antibody.
Limitation:
This 17HSD7 antibody is available for research use only.
Localization:
Cytoplasmic (ER)
Purity:
Antigen affinity purified
Uniprot #:
P56937