VPS36 Antibody / Eap45

NSJ Bioreagents
Product Code: NSJ-RQ7384
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7384-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the VPS36 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human PC-3 cells with VPS36 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VPS36 antibody.~
2 / 2
Western blot testing of 1) human HL60, 2) human ThP-1, 3) human MOLT4, 4) hu

Flow cytometry testing of human PC-3 cells with VPS36 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VPS36 antibody.~
Western blot testing of 1) human HL60, 2) human ThP-1, 3) human MOLT4, 4) hu

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the VPS36 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Vacuolar protein-sorting-associated protein 36 is a protein that in humans is encoded by the VPS36 gene. This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-S386) was used as the immunogen for the VPS36 antibody.
Limitation:
This VPS36 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q86VN1