DHX29 Antibody / DEAH box protein 29

NSJ Bioreagents
Product Code: NSJ-RQ7385
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7385-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the DHX29 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with DHX29 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DHX29 antibody.~
2 / 2
Western blot testing of human 1) HeLa, 2) 293T and 3) HT1080 cell lysate wit

Flow cytometry testing of human 293T cells with DHX29 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DHX29 antibody.~
Western blot testing of human 1) HeLa, 2) 293T and 3) HT1080 cell lysate wit

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DHX29 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ATP-dependent RNA helicase DHX29, also called DEAH-box helicase 29, is a 155 kDa protein that in humans is encoded by the DHX29 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R56-K1343) was used as the immunogen for the DHX29 antibody.
Limitation:
This DHX29 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q7Z478