HSD17B8 Antibody / 17-beta-hydroxysteroid dehydrogenase 8

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NSJ Bioreagents
Product Code: NSJ-RQ7388
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7388-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the HSD17B8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human JK cells with HSD17B8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSD17B8 antibody.~
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Western blot testing of 1) human HepG2, 2) human HeLa, 3) rat liver and 4)
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Flow cytometry testing of human JK cells with HSD17B8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSD17B8 antibody.~
Western blot testing of 1) human HepG2, 2) human HeLa, 3) rat liver and 4)

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HSD17B8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Estradiol 17 beta-dehydrogenase 8, also called 17-beta-HSD 8 and Protein Ke6, is an enzyme that in humans is encoded by the HSD17B8 gene. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D42-D244) was used as the immunogen for the HSD17B8 antibody.
Limitation:
This HSD17B8 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q92506