B3GNT2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7390
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7390-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the B3GNT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with B3GNT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human 293T, 2) human HL60, 3) human MOLT4, 4) rat brain, 5) rat kidney, 6) mouse brain and 7) mouse kidney tissue lysate with B3GNT2 antibody. Predicted molecular weight ~46 kDa but may be observed at higher molecular weights due to glycosylation.
3 / 4
Flow cytometry testing of human HeLa cells with B3GNT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B3GNT2 antibody.
4 / 4
Flow cytometry testing of human ThP-1 cells with B3GNT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B3GNT2 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with B3GNT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HL60, 3) human MOLT4, 4) rat brain, 5) rat kidney, 6) mouse brain and 7) mouse kidney tissue lysate with B3GNT2 antibody. Predicted molecular weight ~46 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human HeLa cells with B3GNT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B3GNT2 antibody.
Flow cytometry testing of human ThP-1 cells with B3GNT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B3GNT2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the B3GNT2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 is an enzyme that in humans is encoded by the B3GNT2 gene. This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R6-C397) was used as the immunogen for the B3GNT2 antibody.
Limitation:
This B3GNT2 antibody is available for research use only.
Localization:
Cytoplasmic (Golgi)
Purity:
Antigen affinity purified
Uniprot #:
Q9NY97