ERGIC1 Antibody / ERGIC-32

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NSJ Bioreagents
Product Code: NSJ-RQ7397
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7397-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ERGIC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-87 MG cells with ERGIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ERGIC1 antibody.
2 / 3
Flow cytometry testing of human MCF7 cells with ERGIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ERGIC1 antibody.
3 / 3
Western blot testing of 1) human HepG2, 2) human U-87 MG, 3) rat brain, 4) rat liver, 5) rat RH35, 6) mouse brain, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with ERGIC1 antibody. Predicted molecular weight ~33 kDa.
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Flow cytometry testing of human U-87 MG cells with ERGIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ERGIC1 antibody.
Flow cytometry testing of human MCF7 cells with ERGIC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ERGIC1 antibody.
Western blot testing of 1) human HepG2, 2) human U-87 MG, 3) rat brain, 4) rat liver, 5) rat RH35, 6) mouse brain, 7) mouse liver and 8) mouse NIH 3T3 cell lysate with ERGIC1 antibody. Predicted molecular weight ~33 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ERGIC1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Endoplasmic reticulum-Golgi intermediate compartment protein 1 is an endoplasmic reticulum-golgi intermediate compartment (ERGIC) protein which interacts with other members of this protein family to increase their turnover.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S42-H290) was used as the immunogen for the ERGIC1 antibody.
Limitation:
This ERGIC1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q969X5