ANXA10 Antibody / Annexin A10

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NSJ Bioreagents
Product Code: NSJ-RQ7403
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7403-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ANXA10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE human thyroid cancer tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 7
IHC staining of FFPE human lung adenocarcinoma tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human placenta tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human appendiceal adenocarcinoma tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Western blot testing of human 1) Caco-2, 2) HeLa, 3) A431 and 4) K562 cell lysate with ANXA10 antibody. Predicted molecular weight ~37 kDa.
6 / 7
Flow cytometry testing of human PC-3 cells with ANXA10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANXA10 antibody.
7 / 7
Immunofluorescent staining of FFPE human placental tissue with ANXA10 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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IHC staining of FFPE human thyroid cancer tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendiceal adenocarcinoma tissue with ANXA10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) HeLa, 3) A431 and 4) K562 cell lysate with ANXA10 antibody. Predicted molecular weight ~37 kDa.
Flow cytometry testing of human PC-3 cells with ANXA10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANXA10 antibody.
Immunofluorescent staining of FFPE human placental tissue with ANXA10 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ANXA10 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ANXA10 (Annexin A10) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. By FISH, Morgan et al.(1999) mapped the ANXA10 gene to chromosome 4q33. Morgan et al.(1999) suggested that the ablation or depletion of principal calcium-binding sites in annexins A9 and A10, respectively, may dispose them to function in distinct tissue environments where calcium sensitivity is not a major functional determinant.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N49-Y324) was used as the immunogen for the ANXA10 antibody.
Limitation:
This ANXA10 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9UJ72