SARS-CoV-2 (COVID-19) Spike L452R Antibody (Delta Variant)

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ProSci
Product Code: PSI-9463
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9463-0.02mg0.02mg£150.00
Quantity:
PSI-9463-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, 9463, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
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<strong>Figure 2 WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, 9463, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Delta Variant Spike antibody (9463) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
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<strong>Figure 1 SARS-Cov-2 Spike L452R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, 9463, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
<strong>Figure 2 WB Validation of of Delta Variant Spike Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Delta Variant Spike antibody, 9463, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Delta Variant Spike antibody (9463) specifically detects Delta variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>

Documents

Further Information

Additional Names:
SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
WB: 1 μg/mL.

Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike L452R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant (B.1.617.2) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 proteins by ELISA. All other applications and species not yet tested.
Background:
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Background References:
  • Zhang et al. JAMA 2021, 325 (13): 1324-26.
  • Reardon. Nature 2021.
  • Delphine. Nature 2021, 596 (7871): 276-280.
Buffer:
SARS-CoV-2 Spike Delta Variant antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (77.8%)
Immunogen:
Anti-SARS-CoV-2 Spike L452R (Delta Variant) antibody (9463) was raised against a peptide corresponding to 9 amino acids in the receptor binding domain of SARS-CoV-2 Delta variant (B.1.617.2) Spike protein.

The immunogen is located within 430-480 amino acids of SARS-CoV-2 Delta variant (B.1.617.2) Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
Anti-SARS-CoV-2 Spike Delta Variant antibody is affinity chromatography purified via peptide column.
Specificity:
It can only detect SARS-CoV-2 Delta Variant (B.1.617.2) Spike S1 protein and does not cross-react with the spike protein of other variants.
Swissprot #:
P0DTC2
User Note:
Optimal dilutions for each application to be determined by the researcher.