IRGB10 (CT) Antibody

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ProSci
Product Code: PSI-9515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9515-0.02mg0.02mg£150.00
Quantity:
PSI-9515-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation In Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: IRGB10 9515, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with 9515 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 Western Blot Validation In Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: IRGB10 9515, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with 9515 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
IRGB10 Antibody: Immunity-related GTPase family member b10, Interferon-gamma-inducible p47 GTPase, Gm12250, OTTMUSG00000005723
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
IRGB10 Antibody: IRGB10 is an inferno-inducible protein that is essential for the activation of DNA-sensing AIM2 inflammasome, and contributes to the activation of LPS-sensing caspase-11 and NLRP3 inflammasome. IRGB10 is part of a conserved signaling hub at the interface between cell-autonomous immunity and innate immune sensing pathways. IRGB10 targets cytoplasmic bacteria and compromises bacterial structural integrity. IRGB10 and LIGP2 act together to confer differential susceptibility against Chlamydia infection in mice.
Background References:
  • Man et al. Cell 2016; 167(2):382-396.e17
  • Bernstein-Hanley I et al. Proc Natl Acad Sci USA. 2006;103(38):14092-14097
  • Miyairi I et al. J Immunol 2007; 179(3):1814-1824
  • Coers J et al. J Immunol 2008; 180(9):6237-6245
Buffer:
IRGB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Monkey (100%), Gorilla (100%)
Immunogen:
Anti-IRGB10 antibody (9515) was raised against a peptide corresponding to 13 amino acids near the C-terminus of mouse IRGB10.

The immunogen is located within the last 50 amino acids of IRGB10.
Isoforms:
Mouse IRGB10 has 1 isoform (417aa, 47.2kD).
Molecular Weight:
Predicted: 47kD

Observed: 47 kD
NCBI Gene ID #:
631323
NCBI Official Name:
predicted gene 12250
NCBI Official Symbol:
IRGB10
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
AGY29631
Protein GI Number:
551032829
Purification:
IRGB10 Antibody is affinity chromatography purified via peptide column.
Swissprot #:
AGY29631
User Note:
Optimal dilutions for each application to be determined by the researcher.