PGAM5 (CT) Antibody

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ProSci
Product Code: PSI-9517
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9517-0.02mg0.02mg£150.00
Quantity:
PSI-9517-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4 °C for three months and -20 °C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation in Mouse and Rat Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 Western Blot Validation in Rat Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 5 Immunofluorescence Validation of PGAM5 in Human Liver</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of PGAM5 in Mouse Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with 9517 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 7 Immunofluorescence Validation of PGAM5 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 Western Blot Validation in Mouse and Rat Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Western Blot Validation in Rat Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 5 Immunofluorescence Validation of PGAM5 in Human Liver</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PGAM5 in Mouse Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with 9517 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PGAM5 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5
Application Note:
WB: 0.5 μg/mL; IF: 10-20 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
PGAM5 Antibody: PGAM5 is a central mediator for programmed necrosis induced by TNF and may be a regulator of mitochondrial dynamics. It displays phosphatase activity for serine/threonine residues, dephosphorylates and activates MAP3K5 kinase. It has apparently no phosphoglycerate mutase activity and is a substrate for a KEAP1-dependent ubiquitin ligase complex. It is also contributes to the repression of NFE2L2-dependent gene expression
Background References:
  • Lo S. et al. Exp. Cell Res. 2018; 314(8): 1789-1803
  • Takeda et al. PNAS 2009; 106(30): 12301-12305
  • Want et al. Cell 2012; 148(1-2); 228-243
Buffer:
PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: gorilla (100%); Chimpanzee (100%); monkey (100%).
Immunogen:
Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5.

The immunogen is located within the last 50 amino acids of PGAM5.
Isoforms:
Human PGAM5 has 2 isoforms, including isoform 1 (289aa, 32kD), and isoform 2 (255aa, 28kD). Mouse PGAM5 has 2 isoforms, including isoform 1 (288aa, 32kD) and isoform 2 (287aa, 31.9kD). Rat PGAM5 has one isoform (288aa, 32kD). 9517 can detect PGAM5 in human, mouse and rat
Molecular Weight:
Predicted: 32kD

Observed: 32 kD
NCBI Gene ID #:
192111
NCBI Official Name:
PGAM family member 5, mitochondrial serine/threonine protein phosphatase
NCBI Official Symbol:
PGAM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q96HS1
Protein GI Number:
150417955
Purification:
PGAM5 Antibody is affinity chromatography purified via peptide column.
Swissprot #:
Q96HS1
User Note:
Optimal dilutions for each application to be determined by the researcher.