SARS-CoV-2 (COVID-19) Spike 156-157EF Antibody

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ProSci
Product Code: PSI-9685
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9685-0.02mg0.02mg£150.00
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PSI-9685-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike 156-157EF antibody (9685) at 0.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. <strong>Strong spike protein signal was observed in the WT COVID-19 patient lung, but not in Delta variant COVID-19 patient lung. </strong>
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<strong>Figure 2 SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, 9685, 1 μg/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.
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<strong>Figure 3 WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, 9685, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 156-157EF antibody (9685) detects spike S1 protein of WT, but not Delta variant. </strong>
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<strong>Figure 1 Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike 156-157EF antibody (9685) at 0.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. <strong>Strong spike protein signal was observed in the WT COVID-19 patient lung, but not in Delta variant COVID-19 patient lung. </strong>
<strong>Figure 2 SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, 9685, 1 μg/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.
<strong>Figure 3 WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, 9685, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 156-157EF antibody (9685) detects spike S1 protein of WT, but not Delta variant. </strong>

Documents

Further Information

Additional Names:
SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
WB: 1 μg/mL; IHC: 0.5 μg/mL.

Antibody validated: Western Blot and IHC in human samples. Anti-SARS-CoV-2 Spike 156-157EF antibody specifically does not detect SARS-CoV-2 Delta Variant (B.1.617.2) Spike S1 protein, but detects Spike S1 protein of WT and other variants by ELISA. All other applications and species not yet tested.
Background:
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Background References:
  • Zhang et al. JAMA 2021, 325 (13): 1324-26.
  • Reardon. Nature 2021.
  • Delphine. Nature 2021, 596 (7871): 276-280.
Buffer:
SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (38.5%)
Immunogen:
Anti-SARS-CoV-2 Spike 156-157EF antibody (9685) was raised against a peptide corresponding to 13 amino acids near the amino terminal domain of SARS-CoV-2 Spike protein.
The immunogen is located within 130-180 amino acids of SARS-CoV-2 Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
Anti-SARS-CoV-2 Spike 156-157EF antibody is affinity chromatography purified via peptide column.
Swissprot #:
P0DTC2
User Note:
Optimal dilutions for each application to be determined by the researcher.