HIV-1 p24 Antibody [2C2C12] (biotin)

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ProSci
Product Code: PSI-PM-7365-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7365-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7365-biotin-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2C2C12
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation with HIV-1 p24 Protein </strong><br> Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7365, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.
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<strong>Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein </strong><br> Coating Antigen: HIV-1 p24 recombinant protein, 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7365, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
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<strong>Figure 1 Western Blot Validation with HIV-1 p24 Protein </strong><br> Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7365, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.
<strong>Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein </strong><br> Coating Antigen: HIV-1 p24 recombinant protein, 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7365, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.

Documents

Further Information

Additional Names:
Anti-HIV-1 p24 antibody: Pr55Gag, Gag polyprotein, Capsid protein p24
Application Note:
WB: 1 μg/mL.
Anti-HIV-1 p24 antibody detects HIV-1 p24 protein by ELISA and WB. All other applications and species not yet tested.
Background:
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core, and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 protein has been used as an integral part of multicomponent HIV-1 vaccines (3).
Background References:
  • Goto et al. Micron 1998; 29:123-38.
  • Freed EO. Virology 1998; 251:1-15.
  • Freed EO. Virology 1998; 251:1-15.
Buffer:
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
Immunogen:
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
NCBI Gene ID #:
155030
NCBI Official Name:
gag Pr55(Gag)
NCBI Official Symbol:
GAG
NCBI Organism:
hiv-1
Physical State:
Liquid
Predicted Molecular Weight:
24 kDa
Protein Accession #:
AAB50258
Protein GI Number:
327745
Purification:
Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
Swissprot #:
P04591
User Note:
Optimal dilutions for each application to be determined by the researcher.