SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]

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ProSci
Product Code: PSI-PM-9583
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-9583-0.02mg0.02mg£150.00
Quantity:
PSI-PM-9583-0.1mg0.1mg£515.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 1C3H9
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
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<strong>Figure 2 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein. </strong>
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<strong>Figure 3 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1). Detection Antibodies: Spike 26P antibody, PM-9583, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein. </strong>
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<strong>Figure 4 ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide </strong><br> Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 spike 26P antibody detects WT peptide (26P, 9083P), but not Gamma variant spike S1 peptide (26S, 9573P). </strong>
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<strong>Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
<strong>Figure 2 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein. </strong>
<strong>Figure 3 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1). Detection Antibodies: Spike 26P antibody, PM-9583, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein. </strong>
<strong>Figure 4 ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide </strong><br> Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 spike 26P antibody detects WT peptide (26P, 9083P), but not Gamma variant spike S1 peptide (26S, 9573P). </strong>

Documents

Further Information

Additional Names:
SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
WB: 1 μg/mL.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Background:
In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named Gamma variant, was discovered in Japan and later spread in Brazil. It is considered a VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.
Background References:
  • Voloch et al. Journal of Virology 2021, 95 (10): e00119-21.
  • Wang et al. Cell Host & Microbe 2021, 29(5): 747-751.
  • Wang et al. Nature 592, 616-622.
Buffer:
SARS-CoV-2 Spike 26P antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
Immunogen:
Anti-SARS-CoV-2 Spike 26P antibody (PM-9583) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Spike protein.
The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
Anti-SARS-CoV-2 Spike 26P antibody is Protein A purified.
Specificity:
It detects Spike S1 proteins of SARS-CoV-2 WT and variants except gamma variant.
Swissprot #:
P0DTC2
User Note:
Optimal dilutions for each application to be determined by the researcher.