SARS-CoV-2 (COVID-19) Spike P26S Antibody [5G12G11] (Gamma Variant)

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ProSci
Product Code: PSI-PM-9590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-9590-0.02mg0.02mg£150.00
Quantity:
PSI-PM-9590-0.1mg0.1mg£515.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5G12G11
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
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<strong>Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
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<strong>Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, PM-9590, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
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<strong>Figure 4 ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide </strong><br> Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S, 9573P), but not WT peptide (26P, 9083P). </strong>
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<strong>Figure 1 SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
<strong>Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
<strong>Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein </strong><br> Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, PM-9590, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300). </strong>
<strong>Figure 4 ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide </strong><br> Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. <strong>SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S, 9573P), but not WT peptide (26P, 9083P). </strong>

Documents

Further Information

Additional Names:
SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
WB: 1 μg/mL.
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
Background:
In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named Gamma variant, was discovered in Japan and later spread in Brazil. It is considered a VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.
Background References:
  • Voloch et al. Journal of Virology 2021, 95 (10): e00119-21.
  • Wang et al. Cell Host & Microbe 2021, 29(5): 747-751.
  • Wang et al. Nature 592, 616-622.
Buffer:
SARS-CoV-2 Spike P26S (Gamma Variant) antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (36.4%)
Immunogen:
Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (PM-9590) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein.
The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Purification:
Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody is immunoaffinity chromatography purified IgG.
Specificity:
It can only detect SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein and does not cross-react with the spike protein of other variants.
Swissprot #:
P0DTC2
User Note:
Optimal dilutions for each application to be determined by the researcher.