AO solution
Code | Size | Price |
---|
U0555-1mL | 1 mL | £195.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
Storage:
Store at -20°C. Protect from light.
Further Information
Form:
Liquid
Product Description:
Acridine orange (AO) solution is used as nuclear dye. AO forms a complex with double-stranded DNA to emit green fluorescence, AO also forms a complex with singlestranded DNA or RNA to emit red fluorescence. One molecule of AO intercalates with three base pairs of double-stranded DNA and emits green fluorescence with the maximum wavelength at 526 nm (excitation 502 nm). One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked, structure that emits red fluorescence with the maximum wavelength at 650 nm (excitation 460 nm)