His tag monoclonal antibody, clone 33D10.D2.G8 (HRP)
| Code | Size | Price |
|---|
| MAB23676-100ug | 100 ug | £709.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 33D10.D2.G8
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Further Information
Concentration:
1.0 mg/mL by UV abso
Conjugate Tag:
HRP
Form:
Lyophilized
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Immunogen Sequence:
HHHHHH
Product Description:
Mouse monoclonal antibody raised against synthetic peptides of His tag.
The antibody is conjugated with horseradish peroxidase (HRP).
The antibody is conjugated with horseradish peroxidase (HRP).
Recommended Dilutions:
ELISA (1:10,000)
Immunohistochemistry (1:500 - 1:2,500)
Western Blot (1:1,000 - 1:5,000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500 - 1:2,500)
Western Blot (1:1,000 - 1:5,000)
The optimal working dilution should be determined by the end user.
Specificity:
The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Storage Buffer:
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (with 10 mg/mL BSA [immunoglobulin and protease free) and 0.01% (w/v) gentamicin sulfate).