Fura-FF (potassium salt)
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Overview
Regulatory Status: RUO
Shipping:
cool pack
Storage:
-20°C
Documents
Further Information
Bioactivity:
Fura-FF is a difluorinated derivative of the calcium indicator fura-2. Unlike, fura-2, fura-FF has negligible magnesium sensitivity, thus reducing interference from this cation.[1] Fura-FF also has a higher calcium dissociation constant than fura-2 (Kd(calcium) = 6 and 0.14 uM, respectively).[1],[2]However, the spectral properties of fura-FF and fura-2 are similar with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium, with a shift to 339/507 nm in the presence of a high calcium concentration.[3] Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high concentrations of calcium, such as mitochondria, or in cell systems that have relatively low calcium buffering capacities, such as neuronal dendrites and spines.[4],[5[,[6]
CAS:
192140-58-2
Molecular Weight:
858.987
Purity:
0.98
SMILES:
[K].[K].[K].[K].[K].OC(=O)CN(CC(O)=O)c1ccc(F)c(F)c1OCCOc1cc2cc(oc2cc1N(CC(O)=O)CC(O)=O)-c1ncc(o1)C(O)=O
References
Refererence:|Hyrc, K.L., Bownik, J.M., and Goldberg, M.P. Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC. Cell Calcium 27(2), 75-86 (2000).|Grynkiewicz, G., Poenie, M., and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260(6), 3440-3450 (1985).|Ruggiu, A.A., Bannwarth, M., and Johnsson, K. Fura-2FF-based calcium indicator for protein labeling. Org. Biomol. Chem. 8(15), 3398-3401 (2010).|Aponte, Y., Bischofberger, J., and Jonas, P. Efficient Ca2+ buffering in fast-spiking basket cells of rat hippocampus. J.Physiol. 586(8), 2061-2075 (2008).|Canepari, M., Vogt, K., and Zecevic, D. Combining voltage and calcium imaging from neuronal dendrites. Cell.Mol.Neurobiol. 28(8), 1079-1093 (2008).|Marcu, R., Neely, C.K., Karamanlidis, G., et al. Multi-parameter measurement of the permeability transition pore opening in isolated mouse heart mitochondria. J.Vis.Exp. 67, (2012).