Anti-Human/Mouse/Rat Glycophorin A - Purified
Code | Size | Price |
---|
LEI-G210-25ug | 25 ug | £127.00 |
Quantity:
LEI-G210-100ug | 100 ug | £171.00 |
Quantity:
LEI-G210-500ug | 500 ug | £331.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 κ
Antibody Clonality: Monoclonal
Antibody Clone: JC159
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Functional Study
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
2-8°C
Storage:
This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
Glycophorin A (CD235a) is expressed on hematopoietic stem cells,
erythroid cells, and erythrocytes. Erythroleukemia can express glycophorin A on neoplastic
erythroblasts.
Concentration:
0.5 mg/ml
Conjugate/Tag/Label:
Purified No Carrier Protein
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Immunogen:
Membrane preparation from splenic hairy cell leukemia
Long Description:
Glycophorins are transmembrane proteins of red blood cells (RBC)1 . There are four glycophorin encoding genes (A, B, C, and D), and a fifth pseudogene (E), which has no protein product.
Glycophorin A, B, and E are tandemly arranged repeats on chromosome 4 and are prone to
rearrangements that lead to rare blood groups. Glycophorin A and B carry MN and Ss blood group antigens.
Glycophorin A is a type I glycoprotein carrying sialylated O- and N-glycans, and most of the O-glycans are NeuAcα2-3Galβ1-3(NeuAcα2-6)GaINAc-tetrasaccharide chains linked to serine or
threonine residues 2 . The abundance of glycophorin A on the surface of RBCs is thought to create
a negatively charged cell surface of complex glycans that allows for RBC circulation without
sticking to other cells or blood vessel walls1 .
Glycophorin A binds CD170 3 (Siglec 5, which mediates sialic-acid dependent binding to cells4),
influenza virus, and EBA-175, the erythrocyte binding antigen of Plasmodium falciparum, the
parasite responsible for malaria in Africa 2 . Babesia divergens, which like Plasmodium is a
parasite in the Apicomplexa phylum, also uses glycophorins A and B to enter RBCs. Other
pathogens that bind to glycophorin A on the surface of RBCs include: some strains of
Escherichia coli, reoviruses (a group of viruses that includes rotavirus), encephalomyocarditis virus, and hepatitis A 2 . Glycophorin A also interacts with influenza virus but because influenza virus cannot replicate in anucleated RBCs glycophorin A might be acting as a decoy receptor to divert infection away from other tissues.
JC159 was generated using splenic hairy cell leukemia cells as immunogen and reacts with a
formalin-fixed, paraffin-embedded resistant epitope, likely between amino acids 27 and 40, on
the extracellular domain5.
NCBI Gene:
2993
Target:
Glycophorin A
References
1 Hollox EJ, Louzada S. 75(3):201-206. 2023.
2 Jaskiewicz E, Jod?owska M, Kaczmarek R, et al. Parasit Vectors. 12(1):317. 2019.
3 https://apps.vetmed.wsu.edu/tkp/report.aspx?ID=311
4 https://www.uniprot.org/uniprotkb/O15389/entry
5 Erber WN, McLachlan J, Cordell JL, et al. Hematology Reviews and Communications.
5(2):113-120. 1991.
6 Tommila M, Stark C, Jokilammi A, et al. J Mol Cell Biol. 3(3):190-196. 2011.
7 Sadahira Y, Kanzaki A, Wada H, et al. J Clin Pathol. 52(12):919-921. 1999.
8 Sutherland DR, Keeney M, Illingworth A. Cytometry B Clin Cytom. 82(4):195-208. 2012.
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