Anti-Mouse/Rat CD90.1 (Thy-1.1) (Clone OX-7) - Purified in vivo PLATINUM™ Functional Grade
| Code | Size | Price |
|---|
| LEI-C852-1.0mg | 1.0 mg | £283.00 |
Quantity:
| LEI-C852-5.0mg | 5.0 mg | £456.00 |
Quantity:
| LEI-C852-25mg | 25 mg | £1,335.00 |
Quantity:
| LEI-C852-50mg | 50 mg | £1,938.00 |
Quantity:
| LEI-C852-100mg | 100 mg | £2,741.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: OX-7
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Applications:
- Depletion
- Flow Cytometry
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Further Information
Antigen Distribution:
CD90 is present on a variety of cell types in murine and rat, including lymphatic vessels1, T cells2, neurons3 and fibroblasts4.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade, in vivo PLATINUM™
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Long Description:
CD90/Thy-1 (murine CD90.1/Thy-1.1) is a cell surface glycoprotein that was the first protein of the immunoglobulin superfamily to be discovered5. The 25 kDa core protein is N-glycosylated at three sites, leading to isoforms with a range of molecular masses (25-37 kDa). CD90 is composed of a single V-like immunoglobulin domain anchored by a disulfide bond between Cys 28 and Cys 104. Despite its lack of an intracellular domain, CD90 is located in the outer leaflet of lipid rafts at the cell membrane, allowing signaling functions by cis- and trans-interactions with a variety of proteins, including G inhibitory proteins, the Src family kinase (SFK) members src and c-fyn, and tubulin.
CD90 has been found to play a role in numerous cellular activities, such as cell adhesion, apoptosis, metastasis, inflammation, and fibrosis. In mouse strains expressing CD90.1, it is expressed on early-stage hematopoietic cells in bone marrow, thymocytes, and circulating mature T cells. The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
NCBI Gene:
21838
Purity:
?98% monomer by analytical SEC, >95% by SDS Page
Target:
CD90.1
References
1. Jurisic G, Iolyeva M, Proulx ST, et. al. Exp Cell Res. 316: 2982?2992. 2010.
2. Ledbetter JA, Rouse RV, Micklem HS, et. al. J Exp Med. 152: 280?295. 1980.
3. Schrader JW, Battye F, Scollay R. Proc Natl Acad Sci. U S A 79: 4161?4165. 1982.
4. Phipps RP, Baecher C, Frelinger JG, et. al. Eur J Immunol. 20: 1723?1727. 1990.
5. Hu P, Leyton L, Hagood JS, Barker TH. Front Cell Dev Biol. Jun 6;10:928510. 2022.
6. Crook K, Hunt SV. Dev Immunol. 4(4):235-46. 1996.
7. Zhang J, Wang JH. Methods Mol Biol. 1842:217-228. 2018
8. Hermans MH, Opstelten D. J Histochem Cytochem. Dec;39(12):1627-34. 1991