Anti-CD157 (BST-1) (Human) mAb

FCM - 5-10 ug/mL per 3 x 105 cells (final concentration)

In the bone marrow (BM) hematopoietic microenvironment, stromal cells play crucial roles. Stromal cells secrete a variety of cytokines and express cell surface molecules that regulate the growth of hematopoietic cells. BM stromal cell lines derived from patients with rheumatoid arthritis (RA) have augmented ability to support the growth of a murine pre-B cell line, DW34. BST-1 (bone marrow stromal cell antigen-1) is expressed on BM stromal cell lines and is responsible for an augmented ability to support pre-B cell line growth. BST-1 is also expressed by RA-derived synovial cell lines, a myelomonocytic cell lines, and HUVEC, suggesting that BST-1 has other functional roles than just supporting pre-B cell growth.

This antibody is lyophilized form.Prepare a stock solution by dissolving the lyophilizedantibody in 100uL of distilled water. After reconstitution,the IgG concentration will be 1 mg/mL in PBS (pH 7.2)/1% sucrose, 0.09% NaN3.*Azide may react with

Human: 683 Mouse: 12182

RASV5-5 bone-marrow stromal cell line derived from RA

This antibody reacts with CD157 antigen on Flow cytometry. This antibody reacts with RASV5-5 cells, and does not react with a healthy donor-derived nonsupportive cell line, NFSV1-1.

1 year

This antibody was purified from hybridoma (clone RF3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3X63Ag8.653 with Balb/c mouse splenocyte immunized with RA-derived BM stromal cell line RASV5-5.

BST-1/CD157