Rat spleen tissue lysate (denatured)
Product Code: L042W1
Product Group: Cell and Tissue Lysates and Immunoblots
Supplier: Abnova Corporation
| Code | Size | Price |
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| L042W1-200ug | 200 ug | £131.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Additional shipping charges apply. Please contact us for a quote. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Regulatory Status: RUO
Application: Western Blot (WB)
Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Further Information
Antigen Target Species:
Rat
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method:
Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Product Description:
Whole tissue lysate (denatured)
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Recommended Dilutions:
The lysate is ready to load on SDS-PAGE for Western blotting.
Storage Buffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Tissue:
Spleen