Hepa 1-6 (mouse hepatoma) whole cell lysate (non-denatured)
Product Code: L063V2
Product Group: Cell and Tissue Lysates and Immunoblots
Supplier: Abnova Corporation
| Code | Size | Price |
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| L063V2-200ug | 200 ug | £131.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Additional shipping charges apply. Please contact us for a quote. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
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Overview
Host Type: Mouse
Regulatory Status: RUO
Applications:
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Further Information
Antigen Target Species:
Mouse
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Description:
Whole cell lysate (non-denatured)
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Recommended Dilutions:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer:
In modified RIPA Lysis Buffer.
Tissue:
Liver