Nuclei monoclonal antibody, clone 235-1

Human

Liquid

Native puridfied from human cell homogenate.

Mouse monoclonal antibody raised against native Nuclei.

Immunofluorescence (1:100)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.

This antibody reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. Specific for endogenous levels of human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

In culture supernatant.