CD14 monoclonal antibody, clone biG 13

Abnova Corporation
Product Code: MAB5654
Product Group: Primary Antibodies
CodeSizePrice
MAB5654-100ug100 ug£384.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: biG 13
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at -20°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

Further Information

Antigen Target Species:
Human
Form:
Lyophilized
Gene Description:
CD14 molecule
Gene ID:
929
Gene Name:
CD14
Immunogen:
Monocytes of different species and immunoaffinity purified soluble human CD14.
Product Description:
Mouse monoclonal antibody raised against CD14.
Recommend Dilutions:
ELISA
Flow Cytometry
Western Blot
CD14 inhibition studies
The optimal working dilution should be determined by the end user.
Specificity:
biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.
Characteristic: 20 ug/mL inhibits binding of 0.5 ug/mL LPS to CD14, binding epitope amino acid 9-13, 39-44. Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000. Inhibts LPS induced IL1beta and TNF-secretion.
Storage Buffer:
Lyophilized from PBS.

References

  • http://www.ncbi.nlm.nih.gov/pubmed/8612616
  • http://www.ncbi.nlm.nih.gov/pubmed/9030727