ATG8 polyclonal antibody

Yeast

Lyophilized

Atg8p

852200

ATG8

Recombinant protein corresponding to Saccharomyces cerevisiae ATG8.

This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast APG8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Rabbit polyclonal antibody raised against recombinant ATG8.

NP_009475; P38182

ELISA (1:20000-1:100000)
Western Blot (1:4000-1:8000)
The optimal working dilution should be determined by the end user.

This is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.

Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide).