V5 epitope tag polyclonal antibody

Abnova Corporation
Product Code: PAB11383
Product Group: Primary Antibodies
CodeSizePrice
PAB11383-100ug100 ug£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Further Information

Antigen Target Species:
Viruses
Form:
Liquid
Gene Description:
phosphoprotein
Gene ID:
3160800
Gene Name:
V/P
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of Simian virus 5 V5 epitope tag.
Immunogen Sequence Protein Sequence:
GKPIPNPLLGLDST
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Rabbit polyclonal antibody raised against synthetic peptide of V5 epitope tag.
Protein Accession:
YP_138513
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Dilutions:
ELISA (1:10000-1:60000)
Western Blot (1:5000-1:10000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Specificity:
This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts This antibody does not cross-react with endogenous proteins. This antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells.
Storage Buffer:
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)