CASP9 polyclonal antibody

Abnova Corporation
Product Code: PAB14669
Product Group: Primary Antibodies
CodeSizePrice
PAB14669-50uL50 uL£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Immune Electron Microscopy
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Further Information

Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
APAF-3|APAF3|CASPASE-9c|ICE-LAP6|MCH6
Gene Description:
caspase 9, apoptosis-related cysteine peptidase
Gene ID:
842
Gene Name:
CASP9
Immunogen:
Recombinant protein corresponding to human CASP9.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Rabbit polyclonal antibody raised against recombinant CASP9.
Protein Accession:
NP_001220
Recommend Dilutions:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.
Specificity:
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
Storage Buffer:
In serum (0.05% sodium azide)

References

  • http://www.ncbi.nlm.nih.gov/pubmed/10196914
  • http://www.ncbi.nlm.nih.gov/pubmed/16200200