Beta-Galactosidase polyclonal antibody
| Code | Size | Price |
|---|
| PAB15374-1mL | 1 mL | £401.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme Immunoassay (EIA)
- Immunodiffusion (ID)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Escherichia coli
Form:
Liquid
Immunogen:
Recombinant protein corresponding to full length Escherichia coli beta-Galactosidase.
Note:
Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
Preparation Method:
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02 M sodium phosphate, 0.14 M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 um filter, and vialed. No preservative is added.
Product Description:
Goat polyclonal antibody raised against beta-Galactosidase.
Protein Accession:
P00722
Recommend Dilutions:
ELISA (1:400-1:2000)
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.
Specificity:
It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of E. coli.
Storage Buffer:
In 20 mM sodium phosphate buffer, 140 mM NaCl, pH 7.3.