Collagen Type I polyclonal antibody
Code | Size | Price |
---|
PAB10190-100ug | 100 ug | £825.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Human
Form:
Liquid
Immunogen:
Native purified human and bovine placenta Collagen Type I.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Rabbit polyclonal antibody raised against native Collagen Type I.
Recommend Dilutions:
ELISA (1:5000-1:50000)
FLISA (1:100)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:100)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
FLISA (1:100)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:100)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Specificity:
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Storage Buffer:
In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% sodium azide).
References
- http://www.ncbi.nlm.nih.gov/pubmed/14702344
- http://www.ncbi.nlm.nih.gov/pubmed/14722616
- http://www.ncbi.nlm.nih.gov/pubmed/15537655
- https://www.ncbi.nlm.nih.gov/pubmed/33947578