COL1A1 polyclonal antibody
| Code | Size | Price |
|---|
| PAB17204-100uL | 100 uL | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
OI4
Gene Description:
collagen, type I, alpha 1
Gene ID:
1277
Gene Name:
COL1A1
Immunogen:
A synthetic peptide corresponding to human COL1A1.
Product Description:
Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
Protein Accession:
P02452
Recommend Dilutions:
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
Specificity:
This antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. specific to the propeptide portion of the ~180 KDa collagen I alpha1 polypeptide in human lung fibroblast extract. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Storage Buffer:
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
References
- http://www.ncbi.nlm.nih.gov/pubmed/15728585
- http://www.ncbi.nlm.nih.gov/pubmed/2683783