COL1A1 polyclonal antibody

Human

Liquid

OI4

collagen, type I, alpha 1

1277

COL1A1

A synthetic peptide corresponding to amino acids at C-terminus of human COL1A1.

Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.

P02452

Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.

This antibody has been directly tested for reactivity in human, mouse, rat and sheep. Based on the homology of the immunogen this antibody is expected to recognize the collagen I 1 polypeptide in all mammals, birds, and amphibians. specific to the ~ 140 KDa telopeptide portion of the collagen I 1 polypeptide. This antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.

In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)