SMAD3 (phospho S423/425) polyclonal antibody

Product Code: PAB11304

Code	Size	Price

PAB11304-100ug

100 ug

£709.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunohistochemistry (IHC)
Western Blot (WB)

Storage:

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Documents

Supplier External Link

Further Information

Antigen Target Species:

Human

Form:

Liquid

Gene Alias:

DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396

Gene Description:

SMAD family member 3

Gene ID:

4088

Gene Name:

SMAD3

Immunogen:

Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3.

Note:

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Product Description:

Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.

Protein Accession:

NP_005893;P84022

Quality Control Testing:

Antibody Reactive Against Synthetic Peptide.

Recommend Dilutions:

ELISA (1:75000-1:300000)
Western Blot (1:2000-1:20000)
Immunohistochemistry (1:500)
The optimal working dilution should be determined by the end user.

Specificity:

Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.

Storage Buffer:

In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

References

http://www.ijcep.com/files/ijcep0006530.pdf
http://www.ncbi.nlm.nih.gov/pubmed/18922473
http://www.ncbi.nlm.nih.gov/pubmed/12809600
http://www.ncbi.nlm.nih.gov/pubmed/14534577