Nampt (Visfatin/PBEF) (human) (rec.) (His)
Code | Size | Price |
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AG-40A-0018-C050 | 50 ug | £330.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: E.coli
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
-20°C
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Further Information
Alternate Names/Synonyms:
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Biological Activity:
Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
EClass:
32160000
Endotoxin:
<1EU/µg purified protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <1EU/µg purified protein (LAL test; Lonza). Lyophilized. Salt-free. Purity: >90% (SDS-PAGE). Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Molecular Weight:
~52kDa (SDS-PAGE)
NCBI, Uniprot Number:
P43490
Package Type:
Plastic Vial
Product Description:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Purity:
>90% (SDS-PAGE)
Sequence:
Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) | Molecular characteristics of serum visfatin and differential detection by immunoassays: A. Körner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) Lack of direct insulin-like action of visfatin/Nampt/PBEF1 in human adipocytes: E. Wanecq, et al.; J. Physiol. Biochem. 65, 351 (2009) | Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013) | Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is a tumoural cytokine released from melanoma: A.A. Grolla, et al.; Pigment Cell Melanoma Res. 28, 718 (2015)