Flagellin (high purity)
| Code | Size | Price |
|---|
| AG-40B-0025-C010 | 10 ug | £150.00 |
Quantity:
| AG-40B-0025-3010 | 3 x 10 ug | £290.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Salmonella
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Shipping:
Blue Ice
Storage:
-20deg;C
Documents
Further Information
Alternate Names/Synonyms:
FliC
Biological Activity:
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.001EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. Source: Salmonella typhimurium strain 14028. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Binds to human and mouse TLR5 (Toll-like receptor 5). Purity: >95% (SDS-PAGE). Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Molecular Weight:
~54kDa (SDS-PAGE)
NCBI, Uniprot Number:
P06179
Package Type:
Plastic Vial
Product Description:
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Purity:
>95% (SDS-PAGE)
Source / Host:
Salmonella typhimurium strain 14028
Specificity:
Binds to human and mouse TLR5 (Toll-like receptor 5).
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C.
References
Deletion of flagellin's hypervariable region abrogates antibody-mediated neutralization and systemic activation of TLR5-dependent immunity: C. Nempont, et al.; J. Immunol. 181, 2036 (2008) | The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst: T.L. Westerman, et al.; PLoS One 13, e0203698 (2018) | Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages: C.M. Tsai, et al.; J. Innate Immun. 12, 333 (2019) | hGBP1 Coordinates Chlamydia Restriction and Inflammasome Activation through Sequential GTP Hydrolysis: A. Xavier, et al.; Cell Rep. 31, 107667 (2020)