ELISA Kit for Circadian Locomoter Output Cycles Protein Kaput (CLOCK)

KAT13D; bHLHe8; Class E basic helix-loop-helix protein 8

3h

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

0.156-10ng/mL

48T, 96T, 96T?5, 96T?10, 96T?100

Circadian Locomoter Output Cycles Protein Kaput

Double-antibody Sandwich

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Signal transduction;

Tissue homogenates, cell lysates and other biological fluids

The minimum detectable dose of this kit is typically less than 0.063ng/mL

This assay has high sensitivity and excellent specificity for detection of Circadian Locomoter Output Cycles Protein Kaput (CLOCK).
No significant cross-reactivity or interference between Circadian Locomoter Output Cycles Protein Kaput (CLOCK) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Circadian Locomoter Output Cycles Protein Kaput (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Circadian Locomoter Output Cycles Protein Kaput (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

O08785